For pricing in CAD please contact us

SCL-70 - 0.2mg

SCL-70 - 0.2mg

Regular price
$705.47 USD
Regular price
Sale price
$705.47 USD
Unit price
Sold out

DNA topoisomerase I

Uniprot ID:  F1MN93
mRNA RefSeq:  NM_001206487
Protein RefSeq:  NP_001193416
Size: 0.2mg
Source: Calf Thymus
Catalogue Number: ATS01-02

Product Information

Scleroderma (progressive systemic sclerosis) is a systemic autoimmune disease characterised by collagen deposition and connective tissue destruction of the skin, blood vessels and certain internal organs. The prevalence of scleroderma has been estimated to be 1 per 5000. Approximately 20-28% of scleroderma patients have autoantibodies to a nuclear protein referred to as Scl-70. Anti-Scl-70 antibodies are found with a greater frequency in systemic sclerosis patients with proximal skin involvement than in systemic sclerosis patients with limited skin involvement.

The molecular weight of enzymatically-active topoisomerase I determined by SDS-electrophoresis has been reported to range from 67-100 kDa. Recent data suggest that the 100 kDa form is the predominant form in vivo, and that the lower molecular weight forms are due to proteolytic degradation. Both the native and lower molecular weight forms have been shown to effectively bind anti-Scl-70 autoantibodies.

The amino acid sequence of human Scl-70 antigen (DNA topoisomerase I) has recently been described after cloning and sequencing its cDNA. The deduced molecular weight of the mature protein is 90,649 kDa; lower than the SDS-electrophoretic molecular weight. This may be due to post-translational processing or to anomalous electrophoretic behaviour of the protein. The protein was found to be very basic, with a calculated isoelectric point of 10.05, and to possess its catalytic activity within a 67.7 kDa C-terminal fragment. 

PDF-logo-dl Scl-70 datasheet

Clinical Indications



PDF-logo-dl  Material Safety Data Sheet


Medsger, T.A. Jr. (1985) in Arthritis and Allied Conditions, 10thEd. (McCarty, D.J. ed.), pp 994-1036, Lea & Febiger, Philadelph.

Steen, V.D. & Medsger, T.A. Jr. (1990) Rheum. Dis. Clin. North Am. 16, 1

Douvas, A.S. et al. (1979) J. Biol. Chem. 254, 10514

Steen, V.D. et al. (1988) Arthritis Rheum. 31, 196

Shero, J.H. et al. (1986) Science 231, 737

Guldner, H. et al. (1986) Chromosoma 94, 132

Maul, G.G. et al. (1986) Proc. Natl. Acad. Sci. USA 83, 5145

Schmitt, B. et al. (1984) Eur. J. Biochem. 144, 127

Samuels, D.S. et al. (1994) Mol. Biol. Reports 19, 99

Hildebrandt, S. et al. (1990) Clin. Immunol. Immunopathol. 57, 399

Juarez, C. et al. (1988) Arthritis Rheum. 31, 108

Tsay, G. et al. (1990) J. Rheumatol. 17, 1314

Seelig, H.P. et al. (1993) J. Immunol. Methods 165, 241