{"product_id":"sec61a1-polyclonal-antibody-bs74642","title":"SEC61A1 Polyclonal Antibody-BS74642","description":"\u003cp\u003eSEC61A1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS74642-50, BS74642-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P61619\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene belongs to the SECY\/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 52kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of A-549 cells, using SEC61A1 Antibody at 1:3000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Enhanced Kit.\u003cbr\u003e\n Exposure time: 90s., Immunofluorescence analysis of HeLa cells using SEC61A1 Rabbit pAb at dilution of 1:350. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH\/3T3 cells using SEC61A1 Rabbit pAb at dilution of 1:350. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using SEC61A1 Rabbit pAb at dilution of 1:350. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: SEC61A1; HNFJ4; HSEC61; SEC61; SEC61A\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human SEC61A1(NP_037468.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044074881203,"sku":"BS74642-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044074913971,"sku":"BS74642-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e70e4efd-d0ec-4527-a4c8-13469ea9ce0c.jpg?v=1707739602","url":"https:\/\/afsbio.com\/products\/sec61a1-polyclonal-antibody-bs74642","provider":"AFSBio Inc.","version":"1.0","type":"link"}