{"product_id":"securin-monoclonal-antibody-mb11577","title":"Securin Monoclonal Antibody-MB11577","description":"\u003cp\u003eSecurin Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB11577-50, MB11577-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: O95997\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-P, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IP: 1\/20\u003c\/p\u003e  \n\n\u003cp\u003eBackground: Regulatory protein, which plays a central role in chromosome stability, in the p53\/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase\/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 22 kDa; Observed MW: 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Securin in K562, 3T3, Hela lysates using Securin antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using Securin antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: PTTG1; EAP1; PTTG; TUTR1; Securin; Esp1-associated protein; Pituitary tumor-transforming gene 1 protein; Tumor-transforming protein 1; hPTTG\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human Securin\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42975255396531,"sku":"MB11577-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42975255429299,"sku":"MB11577-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_278872cd-1003-4e37-9721-e49408d5fe69.jpg?v=1705662385","url":"https:\/\/afsbio.com\/products\/securin-monoclonal-antibody-mb11577","provider":"AFSBio Inc.","version":"1.0","type":"link"}