{"product_id":"separase-phospho-ser801-polyclonal-antibody-ra29729","title":"Separase (phospho Ser801) Polyclonal Antibody - RA29729","description":"\u003cp\u003eSeparase (phospho Ser801) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: RA29729-50, RA29729-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: extra spindle pole bodies like 1, separase (ESPL1) Homo sapiens Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]) .[supplied by OMIM, Nov 2010],\u003c\/p\u003e\n\n\u003cp\u003eCondition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eStorage Instructions: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eRecommended dilutions: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. Immunofluorescence: 1\/200 - 1\/1000. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC-p; IF\/ICC; ELISA\u003c\/p\u003e\n\n\u003cp\u003eSpecies Cross-Reactivity: Human; Mouse\u003c\/p\u003e\n\n\u003cp\u003eGeneID (Human): 9700\u003c\/p\u003e\n\n\u003cp\u003eProtein MW (KDa): 230\u003c\/p\u003e\n\n\u003cp\u003eSWISS: Q14674\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Reed Bio","offers":[{"title":"50μL","offer_id":45122405925043,"sku":"RA29729-50","price":336.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45122405957811,"sku":"RA29729-100","price":448.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_0e051a7b-e084-4d03-b1bc-2f31f6ebc380.png?v=1773348519","url":"https:\/\/afsbio.com\/products\/separase-phospho-ser801-polyclonal-antibody-ra29729","provider":"AFSBio Inc.","version":"1.0","type":"link"}