{"product_id":"separase-polyclonal-antibody-bt-ap08213","title":"Separase Polyclonal Antibody - BT-AP08213","description":"\u003cp\u003eSeparase Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 20μL, 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BT-AP08213-20, BT-AP08213-50, BT-AP08213-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cell Biology\u003c\/p\u003e\n\n\u003cp\u003eTarget Protein: ESPL1\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-p, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eFull Product Name: Separase Antibody\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189\u003c\/p\u003e\n\n\u003cp\u003eStorage: -20°C for 1 year\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eFormulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight (Da): 233113\u003c\/p\u003e\n\n\u003cp\u003eUniProt Accession: Human: Q14674; Mouse: P60330\u003c\/p\u003e\n\n\u003cp\u003eSynonyms: ESPL1; ESP1; KIAA0165; Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase\u003c\/p\u003e\n\n\u003cp\u003eGeneID: Human: 9700; Mouse: 105988\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"BT Lab","offers":[{"title":"20μL","offer_id":45239899783347,"sku":"BT-AP08213-20","price":151.2,"currency_code":"CAD","in_stock":true},{"title":"50μL","offer_id":45239899816115,"sku":"BT-AP08213-50","price":252.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45239899848883,"sku":"BT-AP08213-100","price":385.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/logo_1bbe0e63-92ea-4e5d-a2a3-2487f0d74e27.png?v=1769400654","url":"https:\/\/afsbio.com\/products\/separase-polyclonal-antibody-bt-ap08213","provider":"AFSBio Inc.","version":"1.0","type":"link"}