{"product_id":"septin-1-polyclonal-antibody-bs77275","title":"Septin 1 Polyclonal Antibody-BS77275","description":"Septin 1 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS77275-50, BS77275-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: Q8WYJ6\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:100   |   IF\/ICC,1:50 - 1:100\n\nBackground: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 48KDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of various lysates, using Septin 1 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 20s., Western blot analysis of Rat spleen, using Septin 1 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 180s., Immunohistochemistry of paraffin-embedded rat spleen using Septin 1 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human appendix using Septin 1 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.\n\nAlternative Name: DIFF6; LARP; PNUTL3; SEP1; SEPT1\n\nImmunogen: Recombinant fusion protein of human Septin 1(NP_443070.5).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047645577395,"sku":"BS77275-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047645610163,"sku":"BS77275-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_78a39e17-6df1-4881-97d8-44432233efcc.jpg?v=1707826718","url":"https:\/\/afsbio.com\/products\/septin-1-polyclonal-antibody-bs77275","provider":"AFSBio Inc.","version":"1.0","type":"link"}