{"product_id":"shc-phospho-y349-polyclonal-antibody-bs4250","title":"Shc (phospho-Y349) Polyclonal Antibody-BS4250","description":"\u003cp\u003eShc (phospho-Y349) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4250-50, BS4250-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P29353\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eShc (phospho-Y349) Polyclonal Antibody-BS4250\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-Shc (Y349) polyclonal antibody detects endogenous levels of Shc protein only when phosphorylated at Tyr349.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 46, 52, 66 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-Shc (Y349) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate\u003cbr\u003e\n Lane 2: HEK293T treated with EGF (100ng\/ml,15 minutes) whole cell lysate\u003cbr\u003e\n Lane 3: HEK293T treated with EGF (100ng\/ml,30 minutes) whole cell lysate\u003cbr\u003e\n Lane 4: The Kidney tissue lysate of Mouse\u003cbr\u003e\n Lane 5: The Kidney tissue lysate of Rat, Immunohistochemistry (IHC) analyzes of p-Shc (Y349) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1; SHC1; SHC; SHCA\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Shc around the phosphorylation site of Tyrosine 349.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957298008243,"sku":"BS4250-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957298041011,"sku":"BS4250-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8914caa1-90dc-48a3-8c13-1c9b8d315002.jpg?v=1705144525","url":"https:\/\/afsbio.com\/products\/shc-phospho-y349-polyclonal-antibody-bs4250","provider":"AFSBio Inc.","version":"1.0","type":"link"}