{"product_id":"simian-fn-fibronectin-elisa-kit-elk11471","title":"Simian FN (Fibronectin) ELISA Kit -ELK11471","description":"\u003cp\u003eSimian FN (Fibronectin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: ELK11471-48, ELK11471-96, ELK11471-96*5\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian FN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian FN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian FN in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: FN1, CIG, FINC, LETS, MSF, GFND2, Anastellin, Migration-Stimulating Factor, Cold-Insoluble Globulin, Large, External, Transformation-Sensitive Protein\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Simian\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal Transduction, Infection Immunity\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.01 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 100 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 1.56-100 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":46136349950131,"sku":"ELK11471-48","price":618.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":46136349982899,"sku":"ELK11471-96","price":882.0,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":46136350015667,"sku":"ELK11471-96*5","price":3748.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/ELK_ELISA_Kit.jpg?v=1790781711","url":"https:\/\/afsbio.com\/products\/simian-fn-fibronectin-elisa-kit-elk11471","provider":"AFSBio Inc.","version":"1.0","type":"link"}