{"product_id":"simian-lep-leptin-elisa-kit-elk11476","title":"Simian LEP (Leptin) ELISA Kit -ELK11476","description":"\u003cp\u003eSimian LEP (Leptin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Number: ELK11476-48, ELK11476-96, ELK11476-96*5\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian LEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian LEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian LEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian LEP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Simian\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic Pathway, Endocrinology, Cardiovascular Biology\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.137 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":46136351654067,"sku":"ELK11476-48","price":397.5,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":46136351686835,"sku":"ELK11476-96","price":564.0,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":46136351719603,"sku":"ELK11476-96*5","price":2397.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/ELK_ELISA_Kit.jpg?v=1790781711","url":"https:\/\/afsbio.com\/products\/simian-lep-leptin-elisa-kit-elk11476","provider":"AFSBio Inc.","version":"1.0","type":"link"}