{"product_id":"skp2-p45-p399-polyclonal-antibody-bs1325","title":"Skp2 p45 (P399) Polyclonal Antibody-BS1325","description":"\u003cp\u003eSkp2 p45 (P399) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1325-50, BS1325-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13309\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B\/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase 2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F, and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: SKP2 p45 (P399) polyclonal antibody detects endogenous levels of SKP2 p45 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of SKP2 at 1:500 dilution\u003cbr\u003e\nLane 1: EC9706 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: HepG2 whole cell lysate (40ug)\u003cbr\u003e\nLane 3: U-87MG whole cell lysate (40ug)\u003cbr\u003e\nLane 4: A375 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Skp2 p45 (P399) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 55 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: S-phase kinase-associated protein 2; Cyclin-A\/CDK2-associated protein p45; F-box protein Skp2; F-box\/LRR-repeat protein 1; p45skp2; SKP2; FBXL1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 369-419 of Human Skp2 p45.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953203843251,"sku":"BS1325-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953203876019,"sku":"BS1325-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_63fb0f93-c04e-4140-a046-7436768bc8bb.jpg?v=1705056696","url":"https:\/\/afsbio.com\/products\/skp2-p45-p399-polyclonal-antibody-bs1325","provider":"AFSBio Inc.","version":"1.0","type":"link"}