{"product_id":"smad3-polyclonal-antibody-bs80100","title":"Smad3 Polyclonal Antibody-BS80100","description":"Smad3 Polyclonal Antibody\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS80100-50, BS80100-100\n\nSwiss-Prot: P84022\n\nHost: Rabbit\n\nSmad3 Polyclonal Antibody-BS80100\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000  |  IF\/ICC,1:50 - 1:200\n\nBackground: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ' mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nSpecificity: Unmodification\n\nBioworld Molecular Weight: 52KDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using at 1:1000 dilution.\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\nLysates\/proteins: 25ug per lane.\nBlocking buffer: 3% non-fat dry milk in TBST.\nDetection: ECL Basic Kit.\nExposure time: 30s., Immunofluorescence analysis of NIH\/3T3 cells using Smad3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\n\nAlternative Name: HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3\n\nImmunogen: Recombinant fusion protein of human Smad3(NP_005893.1).\n\nModification: Unmodified\n\nConjugate: Unconjugated","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42960130998451,"sku":"BS80100-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42960131031219,"sku":"BS80100-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ca50873c-5107-43f3-9df6-25264da45c7f.jpg?v=1705231866","url":"https:\/\/afsbio.com\/products\/smad3-polyclonal-antibody-bs80100","provider":"AFSBio Inc.","version":"1.0","type":"link"}