{"product_id":"smc1a-monoclonal-antibody-mb66490","title":"SMC1A Monoclonal Antibody-MB66490","description":"\u003cp\u003eSMC1A Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66490-50, MB66490-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q14683\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair. ATM\/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint . SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment . While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction .\u003cbr\u003e\nThe serine residue at 360 of SMC1 is phosphorylated in an ATM\/ATR-dependent manner in response to DNA damage . Phospho-SMC1 (Ser360) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS\/MS platform for modification site discovery. Phosphorylation at Ser360 was discovered using an ATM\/ATR substrate antibody and was shown to be induced by UV treatment. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of SMC1A protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 145 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of SMC1A expression in Molt4 (A), U87MG (B), Hela (C) whole cell lysates., Immunofluorescent analysis of SMC1A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Purified recombinant human SMC1A(C-term.) protein fragments expressed in E.coli.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989541589171,"sku":"MB66490-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989541621939,"sku":"MB66490-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8400fa71-854c-4132-9536-5cd9ff93af34.jpg?v=1706095062","url":"https:\/\/afsbio.com\/products\/smc1a-monoclonal-antibody-mb66490","provider":"AFSBio Inc.","version":"1.0","type":"link"}