{"product_id":"smg7-k548-polyclonal-antibody-bs2112","title":"SMG7 (K548) Polyclonal Antibody-BS2112","description":"\u003cp\u003eSMG7 (K548) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2112-50, BS2112-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q92540\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eBackground: In humans, NMD depends on RNA-dependent ATPase and 5' to 3' helicase UPF1, plus six other proteins designated SMG1, SMG5, SMG6, SMG7, UPF2 and UPF3. SMG5, SMG7 and UPF1 localize to cytoplasmic foci called P-bodies, while SMG5, SMG6 and SMG7 target UPF1 for dephosphorylation. SMG7 may also act as an adaptor in targeting mRNAs associated with phosphorylated UPF1 for degradation. SMG7 provides a link between the NMD pathway and mRNA degradation machinery by forming a complex with the proteins SMG5 and UPF1, interacting with them via its N-terminal domain, and targeting bound reporter transcripts for decay via its C-terminal domain. SMG7 contains a 14-3-3-like domain, and residues that bind phosphoserine-containing peptides in 14-3-3 proteins are conserved at the equivalent positions in SMG7.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: SMG7 (K548) polyclonal antibody detects endogenous levels of SMG7 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of SMG7 (K548) pAb at 1:500 dilution\u003cbr\u003e\nLane 1: K562 whole cell lysate (40ug)\u003cbr\u003e\nLane 2: MCF-7 whole cell lysate (40ug)\u003cbr\u003e\nLane 3: U-87MG whole cell lysate (40ug)\u003cbr\u003e\nLane 4: SGC7901 whole cell lysate (40ug)\u003cbr\u003e\nLane 5: PC12 whole cell lysate (40ug)\u003cbr\u003e\nLane 6: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SMG7 (K548) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 127 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: C1orf16; EST1 like protein C; EST1 telomerase component homolog C; EST1C; ever shorter telomeres 1C; FLJ23717; SGA56M; SMG 7;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 512-564 of Human SMG7.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953220260019,"sku":"BS2112-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953220292787,"sku":"BS2112-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_efca22f2-8dc7-4f96-abd8-98162787581b.jpg?v=1705057018","url":"https:\/\/afsbio.com\/products\/smg7-k548-polyclonal-antibody-bs2112","provider":"AFSBio Inc.","version":"1.0","type":"link"}