{"product_id":"smyd4-polyclonal-antibody-bs78344","title":"SMYD4 Polyclonal Antibody-BS78344","description":"\u003cp\u003eSMYD4 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS78344-50, BS78344-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8IYR2\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200   |   IP,1:20 - 1:50\u003c\/p\u003e\n\n\u003cp\u003eBackground: Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1 (By similarity.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: 90kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using SMYD4 antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 60s., Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug SMYD4 antibody. Western blot was performed from the immunoprecipitate using SMYD4 antibody at a dilution of 1:500. Detection: ECL Enhanced Kit.\u003cbr\u003e\n Exposure time: 20s.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: SMYD4; ZMYND21\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human SMYD4(NP_443160.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997524103347,"sku":"BS78344-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997524136115,"sku":"BS78344-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_feed2c81-88e7-47e8-b991-2badfdf4609b.jpg?v=1706270020","url":"https:\/\/afsbio.com\/products\/smyd4-polyclonal-antibody-bs78344","provider":"AFSBio Inc.","version":"1.0","type":"link"}