{"product_id":"sqs-fdft1-polyclonal-antibody-bs7976","title":"SQS\/FDFT1 Polyclonal Antibody-BS7976","description":"\u003cp\u003eSQS\/FDFT1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS7976-50, BS7976-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P37268\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 48KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using SQS\/FDFT1 antibody at 1:500 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Enhanced Kit.\u003cbr\u003e\n Exposure time: 1s., Immunohistochemistry of paraffin-embedded mouse testis using SQS\/FDFT1 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using SQS\/FDFT1 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH-3T3 cells using SQS\/FDFT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: FDFT1; DGPT; ERG9; SQS; SS\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human SQS\/FDFT1(NP_004453.3).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43055866347699,"sku":"BS7976-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43055866380467,"sku":"BS7976-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_e358520b-23a4-41f6-8d1e-3e76358a9dc3.jpg?v=1708085656","url":"https:\/\/afsbio.com\/products\/sqs-fdft1-polyclonal-antibody-bs7976","provider":"AFSBio Inc.","version":"1.0","type":"link"}