{"product_id":"srp20-f151-polyclonal-antibody-bs2559","title":"SRp20 (F151) Polyclonal Antibody-BS2559","description":"\u003cp\u003eSRp20 (F151) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2559-50, BS2559-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P84103\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2\/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A\/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF\/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF\/SF2 and RNA.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: SRp20 (F151) polyclonal antibody detects endogenous levels of SRp20 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 25 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of SRp20 (F151) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: U-87MG whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: Jurkat whole cell lysate (40ug)\u003cbr\u003e\n Lane 3: C6 whole cell lysate (40ug)\u003cbr\u003e\n Lane 4: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of SRp20 (F151) pAb in paraffin-embedded human stomach carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of SRp20 (F151) pAb in paraffin-embedded human liver carcinoma tissue at 1:5000. nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Serine\/arginine-rich splicing factor 3; SFRS3; SRP20; SRSF3\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human SRp20.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42993795727539,"sku":"BS2559-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42993795760307,"sku":"BS2559-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_49a9af59-0a26-44fe-a6c4-91d45a0cfb02.jpg?v=1706183652","url":"https:\/\/afsbio.com\/products\/srp20-f151-polyclonal-antibody-bs2559","provider":"AFSBio Inc.","version":"1.0","type":"link"}