{"product_id":"srrm1-polyclonal-antibody-bs71934","title":"SRRM1 Polyclonal Antibody-BS71934","description":"\u003cp\u003eSRRM1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS71934-50, BS71934-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q8IYB3\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:1000   |   IHC,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B\/SFRS10 and basal snRNP (SNRP70 and SNRPA1 factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 102kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using SRRM1 antibody at 1:3000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 90s., Immunohistochemistry of paraffin-embedded human esophageal cancer using SRRM1 Rabbit pAb at dilution of 1:450. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse testis using SRRM1 Rabbit pAb at dilution of 1:450. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: SRRM1; 160-KD; POP101; SRM160\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human SRRM1(NP_005830.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42999914856627,"sku":"BS71934-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42999914889395,"sku":"BS71934-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_8a6815ff-f584-481a-a1e1-ada58c4a7f49.jpg?v=1706357839","url":"https:\/\/afsbio.com\/products\/srrm1-polyclonal-antibody-bs71934","provider":"AFSBio Inc.","version":"1.0","type":"link"}