{"product_id":"st-5-ht-serotonin-5-hydroxytryptamine-elisa-kit-e-el-0033","title":"ST\/5-HT (Serotonin\/5-Hydroxytryptamine) ELISA Kit - E-EL-0033","description":"\u003cp\u003eST\/5-HT (Serotonin\/5-Hydroxytryptamine) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-EL-0033-24T, E-EL-0033-48T, E-EL-0033-96T\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: ST; 5-HT\u003c\/p\u003e\n\n \n\n\u003cp\u003eTarget Synonyms: 5HT; Enteramine; Thrombocytin; Thrombotonin; 3-(β-Aminoethyl) -5-hydroxyindole\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Universal\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eUsage: This ELISA kit applies to the in vitro quantitative determination of ST\/5-HT concentrations in serum, plasma and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, Plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eType: Competitive\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 2.5h\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 9.38 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 15.63-1000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST\/5-HT. During the reaction, Universal ST\/5-HT in the sample or standard competes with a fixed amount of Universal ST\/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST\/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ST\/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eCross Reactivity: This kit recognizes Universal ST\/5-HT in samples. No significant cross-reactivity or interference between Universal ST\/5-HT and analogues was observed\u003c\/p\u003e\n\n\u003cp\u003eRecovery Rate: 80%-120%\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Both intra-CV and inter-CV are \u0026lt; 10%.\u003c\/p\u003e\n\n\u003cp\u003eStorage: 2-8℃\/-20℃, 12 months\u003c\/p\u003e\n\n\u003cp\u003eShipping: ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"24T","offer_id":43006590877875,"sku":"E-EL-0033-24T","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43006590910643,"sku":"E-EL-0033-48T","price":554.4,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43006590943411,"sku":"E-EL-0033-96T","price":693.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Untitled-1_14a0e4d0-7895-41a1-9d98-4c04dc6fd79e.jpg?v=1706531268","url":"https:\/\/afsbio.com\/products\/st-5-ht-serotonin-5-hydroxytryptamine-elisa-kit-e-el-0033","provider":"AFSBio Inc.","version":"1.0","type":"link"}