{"product_id":"stat1-phospho-y701-polyclonal-antibody-ap0246","title":"Stat1 (Phospho-Y701) Polyclonal Antibody - AP0246","description":"\u003cp\u003eStat1 (Phospho-Y701) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0246-50, AP0246-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The Stat1 transcription factor is activated in response to a large number of ligands and is essential for responsiveness to IFN-α and IFN-γ. Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding. Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors. In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellμlar stresses. Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF, IP\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: Signal transducer and activator of transcription 1-alpha\/beta, Transcription factor ISGF-3 components p91\/p84, STAT1\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylated\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human Stat1 around the phosphorylation site of Tyrosine 701.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB 1:500~1:1000\u003cbr\u003e\nIF: 1:100~1:500\u003cbr\u003e\nIP: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Stat1(Phospho-Y701) polyclonal antibody detects endogenous levels of Stat1 protein only when phosphorylated at Tyr701.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 84 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway(，Inhibition of Apoptosis，Signaling Pathways Activating p38 MAP Kinase\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P42224\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946166083763,"sku":"AP0246-50","price":289.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946166116531,"sku":"AP0246-100","price":492.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/stat1-phospho-y701-polyclonal-antibody-ap0246","provider":"AFSBio Inc.","version":"1.0","type":"link"}