{"product_id":"stra13-polyclonal-antibody-bs71368","title":"STRA13 Polyclonal Antibody-BS71368","description":"\u003cp\u003eSTRA13 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS71368-50, BS71368-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: A8MT69\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: DNA-binding component of the Fanconi anemia (FA core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS (MHF heterodimer, crucial cofactor for FANCM in both binding and ATP-dependent remodeling of DNA. Stabilizes FANCM. In complex with CENPS and FANCM (but not other FANC proteins, rapidly recruited to blocked forks and promotes gene conversion at blocked replication forks. In complex with CENPS, CENPT and CENPW (CENP-T-W-S-X heterotetramer, involved in the formation of a functional kinetochore outer plate, which is essential for kinetochore-microtubule attachment and faithful mitotic progression. As a component of MHF and CENP-T-W-S-X complexes, binds DNA and bends it to form a nucleosome-like structure. DNA-binding function is fulfilled in the presence of CENPS, with the following preference for DNA substates: Holliday junction \u0026gt; double-stranded \u0026gt; splay arm \u0026gt; single-stranded. Does not bind DNA on its own.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of H460 cells, using STRA13 antibody at 1:1000 dilution. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 60s.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 14kDa\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CENPX; CENP-X; D9; FAAP10; MHF2; STRA13\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human STRA13(NP_659435.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42932518289587,"sku":"BS71368-50","price":334.88,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42932518322355,"sku":"BS71368-100","price":576.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a2df1aa2-3a49-46ca-835f-3264e6dfdf47.jpg?v=1704284388","url":"https:\/\/afsbio.com\/products\/stra13-polyclonal-antibody-bs71368","provider":"AFSBio Inc.","version":"1.0","type":"link"}