{"product_id":"sun2-polyclonal-antibody-bs76734","title":"SUN2 Polyclonal Antibody-BS76734","description":"SUN2 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS76734-50, BS76734-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: Q9UH99\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:200\n\nBackground: SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 80kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using SUN2 antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 5s., Immunohistochemistry of paraffin-embedded Human breast using SUN2 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris\/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunofluorescence analysis of C6 cells using SUN2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using SUN2 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.\n\nAlternative Name: SUN2; UNC84B\n\nImmunogen: Recombinant fusion protein of human SUNSUN2\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047640203443,"sku":"BS76734-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047640236211,"sku":"BS76734-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_00df952b-6a79-47a1-89ee-cc7b7cfc3c0f.jpg?v=1707826578","url":"https:\/\/afsbio.com\/products\/sun2-polyclonal-antibody-bs76734","provider":"AFSBio Inc.","version":"1.0","type":"link"}