{"product_id":"synaptotagmin-1-2-phospho-thr202-199-rabbit-polyclonal-antibody-es1461","title":"Synaptotagmin 1\/2 (phospho Thr202\/199) Rabbit Polyclonal Antibody - ES1461","description":"\u003cp\u003eSynaptotagmin 1\/2 (phospho Thr202\/199) Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES1461-50, ES1461-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: SYT1, SVP65, SYT, Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT2, Synaptotagmin-2, Synaptotagmin II, SytII\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human Synaptotagmin around the phosphorylation site of Thr202. AA range:176-225\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: Synaptotagmin 1\/2\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Phospho-Synaptotagmin 1\/2 (T202\/199) Polyclonal Antibody detects endogenous levels of Synaptotagmin 1\/2 protein only when phosphorylated at T202\/199.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/40000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Expressed in melanocytes (PubMed:23999003),\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 60kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Synaptic vesicle cycle\u003c\/p\u003e\n\n\u003cp\u003eFunction: Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains\u003cbr\u003e\nDomain: The first C2 domain mediates Ca(2+)-dependent phospholipid binding\u003cbr\u003e\nDomain: The second C2 domain mediates interaction with SV2A and STN2\u003cbr\u003e\nFunction: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2\u003cbr\u003e\nSimilarity: Belongs to the synaptotagmin family\u003cbr\u003e\nSimilarity: Contains 2 C2 domains\u003cbr\u003e\nSubcellular Location: Synaptic vesicles and chromaffin granules\u003cbr\u003e\nSubunit: H\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Cytoplasmic vesicle, secretory vesicle membrane, Single-pass membrane protein Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass membrane protein Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane, Single-pass membrane protein Cytoplasm\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: SYT1\/SYT2\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: Synaptotagmin-1\/2\u003c\/p\u003e\n\n\u003cp\u003eMouse Gene ID: 20979\/20980\u003c\/p\u003e\n\n\u003cp\u003eRat Gene ID: 25716\/24805\u003c\/p\u003e\n\n\u003cp\u003eRat SwissProt Number: P21707\/P29101\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46315179704499,"sku":"ES1461-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46315179737267,"sku":"ES1461-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies.jpg?v=1793190497","url":"https:\/\/afsbio.com\/products\/synaptotagmin-1-2-phospho-thr202-199-rabbit-polyclonal-antibody-es1461","provider":"AFSBio Inc.","version":"1.0","type":"link"}