{"product_id":"taq-dna-polymerase-antibody-monoclonal-antibody-mb9056","title":"Taq DNA Polymerase Antibody Monoclonal Antibody - MB9056","description":"\u003cp\u003eTaq DNA Polymerase Antibody Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9056-50, MB9056-100\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: Antibody-mediated hot start PCR\u003c\/p\u003e\n\n\u003cp\u003eBackground: Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProG affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze\/thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Taq DNA Polymerase Antibody monoclonal antibody detects endogenous levels of Taq DNA Polymerase Antibody protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Application of the hot start PCR using anti-Taq DNA Polymerase antibody Lane1: Taq DNA Polymerase Lane2: Taq DNA Polymerase + Anti-Taq DNA Polymerase antibody Lane3: Taq DNA Polymerase + Control antibody\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Taq DNA Polymerase\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42892000690355,"sku":"MB9056-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42892000723123,"sku":"MB9056-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b7940adb-0465-43b9-824a-b8a31e701fad.jpg?v=1702468987","url":"https:\/\/afsbio.com\/products\/taq-dna-polymerase-antibody-monoclonal-antibody-mb9056","provider":"AFSBio Inc.","version":"1.0","type":"link"}