{"product_id":"tdg-k90-polyclonal-antibody-bs2053","title":"TDG (K90) Polyclonal Antibody-BS2053","description":"\u003cp\u003eTDG (K90) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS2053-50, BS2053-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13569\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB IHC IF\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003cbr\u003e\nIF: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G\/T mismatches. G\/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G\/T mismatches to G\/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G\/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor\/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2\/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G\/U substrate and the loss of G\/T processing activity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: TDG (K90) polyclonal antibody detects endogenous levels of TDG protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of TDG (K90) pAb at 1:1000 dilution\u003cbr\u003e\nLane1:CT-26 whole cell lysate(40ug)\u003cbr\u003e\nLane2:PC12 whole cell lysate(40ug)\u003cbr\u003e\nLane3:HCT116 whole cell lysate(40ug)\u003cbr\u003e\nLane4:HEK293T whole cell lysate(40ug)\u003cbr\u003e\nLane5:Hela whole cell lysate(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 46 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: G\/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase; Short name=hTDG; Thymine DNA glycosylase; hTDG\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 28-109 of Human TDG.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910477811891,"sku":"BS2053-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910477844659,"sku":"BS2053-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_93540a21-4dd5-414a-b183-7dfd3103290e.jpg?v=1703093217","url":"https:\/\/afsbio.com\/products\/tdg-k90-polyclonal-antibody-bs2053","provider":"AFSBio Inc.","version":"1.0","type":"link"}