{"product_id":"tec-r220-polyclonal-antibody-ap0822","title":"TEC (R220) Polyclonal Antibody-AP0822","description":"\u003cp\u003eTEC (R220) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: AP0822-50, AP0822-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P42680\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:1000~1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Tec kinase belongs to a structurally related subfamily of protein tyrosine kinases (PTKs) that includes Btk, Itk (also known as Emt or Tsk), Bmx, and Txk (or Rlk). With the exception of Txk, the members of this subfamily possess a long amino-terminal region consisting of a pleckstrin homology (PH) domain and a Tec homology (TH) domain . Because PH domains bind phosphoinositides with high affinity, the Tec family kinases have been proposed to act downstream of phosphatidylinositol 3-kinase (PI3-kinase) in signaling pathways. Binding of the PH domain with phosphoinositides is probably required for targeting of Tec family kinases to the cell membrane. Tec kinase is activated in response to many upstream signaling events including antigen receptor, RTK, GPCR, and integrin stimulation. Activated Tec kinase directly phosphorylates substrates such as PLC-gamma 2 and BRDG1 docking protein and mediates downstream signaling.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: TEC (R220) polyclonal antibody detects endogenous levels of TEC protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of TEC (R220) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane1:Myla2059 whole cell lysate(40ug)\u003cbr\u003e\nLane2:K562 whole cell lysate(40ug)\u003cbr\u003e\nLane3:The Spleen tissue lysate of Rat(40ug)\u003cbr\u003e\nLane4:The Spleen tissue lysate of Mouse(40ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 58-75 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Tyrosine-protein kinase Tec; TEC; PSCTK4\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 190-250 of Human TEC.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910473978035,"sku":"AP0822-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910474010803,"sku":"AP0822-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_512e73bc-22d1-4930-873e-568c6665e3cf.jpg?v=1703093126","url":"https:\/\/afsbio.com\/products\/tec-r220-polyclonal-antibody-ap0822","provider":"AFSBio Inc.","version":"1.0","type":"link"}