{"product_id":"tf2a2-polyclonal-antibody-bs62276","title":"TF2A2 Polyclonal Antibody-BS62276","description":"\u003cp\u003eTF2A2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS62276-50, BS62276-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P52657\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG\/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: TF2A2 polyclonal antibody detects endogenous levels of TF2A2 protein.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of TF2A2 polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLaneA:H9C2 whole cell lysate\u003cbr\u003e\nLaneB:AML-12 whole cell lysate\u003cbr\u003e\nLaneC:HEK293T whole cell lysate\u003cbr\u003e\nLaneD:A549 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 17 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Transcription initiation factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; TFIIA-gamma; GTF2A2; TF2A2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to Human TF2A2.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910441046195,"sku":"BS62276-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910441078963,"sku":"BS62276-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_68a4b157-eac1-43fd-bc0d-729682b58eb7.jpg?v=1703092407","url":"https:\/\/afsbio.com\/products\/tf2a2-polyclonal-antibody-bs62276","provider":"AFSBio Inc.","version":"1.0","type":"link"}