{"product_id":"tfiib-monoclonal-antibody-mb0045","title":"TFIIB Monoclonal Antibody-MB0045","description":"\u003cp\u003eTFIIB Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0045-50, MB0045-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG2b, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q00403\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000\u003cbr\u003e\nICC: 1:50~200\u003cbr\u003e\nIHC: 1:500\u003c\/p\u003e \n\n\u003cp\u003eBackground: Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Template commitment is established by the initial binding of TFIID to the “TATA” element of the promotor, a step which may be facilitated by TFIIA. TFIIB then acts as the bridge between TFIID and RNA polymerase II.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 35KDa\u003cbr\u003e\nObserved band size: 37KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of TFIIB in Jurkat, MCF7 and Hela cell lysates using TFIIB antibody (1:1000 diluted)., IHC of paraffin-embedded human colon using anti- TFIIB diluted 1\/500-1\/1000., Immunocytochemistry of HeLa cells using anti- TFIIB antibody diluted 1:50.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: TF2B; TFIIB; S300-II; Transcription initiation factor IIB\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant full length Human TFIIB.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997588197555,"sku":"MB0045-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997588230323,"sku":"MB0045-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_d269dd97-760f-48ab-afe1-d5b8345e50dc.jpg?v=1706270783","url":"https:\/\/afsbio.com\/products\/tfiib-monoclonal-antibody-mb0045","provider":"AFSBio Inc.","version":"1.0","type":"link"}