{"product_id":"thioredoxin-1-trx1-txn-polyclonal-antibody-bs70837","title":"Thioredoxin 1 (Trx1\/TXN) Polyclonal Antibody-BS70837","description":"\u003cp\u003eThioredoxin 1 (Trx1\/TXN) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS70837-50, BS70837-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P10599\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:200   |   IF\/ICC,1:50 - 1:200   |   IP,1:20 - 1:50\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 12kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using Thioredoxin 1) antibody at 1:1000 dilution.\u003cbr\u003e\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\n Lysates\/proteins: 25ug per Lane.\u003cbr\u003e\n Blocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\n Detection: ECL Basic Kit.\u003cbr\u003e\n Exposure time: 90s., Immunohistochemistry of paraffin-embedded human colon using Thioredoxin 1) Rabbit pAb at dilution of 1:300. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of NIH-3T3 cells using Thioredoxin 1) Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug Thioredoxin 1) antibody. Western blot was performed from the immunoprecipitate using Thioredoxin 1) antibody at a dilution of 1:500.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: TXN; TRDX; TRX; TRX1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human (Trx1\/Thioredoxin 1 (Trx1\/TXN))(NP_003320.2).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43055887057075,"sku":"BS70837-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43055887089843,"sku":"BS70837-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_729d6cff-092d-4bbf-b39b-77c922fff469.jpg?v=1708085967","url":"https:\/\/afsbio.com\/products\/thioredoxin-1-trx1-txn-polyclonal-antibody-bs70837","provider":"AFSBio Inc.","version":"1.0","type":"link"}