{"product_id":"thioredoxin-reductase-1-txnrd1-polyclonal-antibody-bs76885","title":"Thioredoxin reductase 1 (TXNRD1 ) Polyclonal Antibody-BS76885","description":"Thioredoxin reductase 1 (TXNRD1 ) Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS76885-50, BS76885-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: Q16881\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IHC, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IHC,1:50 - 1:100   |   IF\/ICC,1:50 - 1:200\n\nBackground: This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 65kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using Thioredoxin reductase 1) antibody at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 90s., Immunohistochemistry of paraffin-embedded human placenta using Thioredoxin reductase 1) antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse testis using Thioredoxin reductase 1) antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of C6 cells using Thioredoxin reductase 1) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\n\nAlternative Name: TXNRD1; GRIM-12; TR; TR1; TRXR1; TXNR\n\nImmunogen: Recombinant fusion protein of human Thioredoxin reductase 1 (TXNRD1)(Q16881).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43047641710771,"sku":"BS76885-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43047641743539,"sku":"BS76885-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_73bb4771-bc31-446b-a1c9-9a0ba1211e1c.jpg?v=1707826604","url":"https:\/\/afsbio.com\/products\/thioredoxin-reductase-1-txnrd1-polyclonal-antibody-bs76885","provider":"AFSBio Inc.","version":"1.0","type":"link"}