{"product_id":"thrombin-r-g17-polyclonal-antibody-bs1856","title":"Thrombin R (G17) Polyclonal Antibody-BS1856","description":"\u003cp\u003eThrombin R (G17) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1856-50, BS1856-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P25116\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Thrombin receptor (also designated protease-activated receptor-1 or PAR-1), PAR-2 and PAR-3 compose a distinct class of G protein-coupled receptors activated by proteolysis. Cleavage of these receptors by proteases occurs within the amino-terminal extracellular domain. Thrombin, a serine protease involved in platelet aggregation and blood coagulation, activates the thrombin receptor, resulting in elevated intracellular calcium levels in platelets. Thrombin also cleaves PAR-3 in vitro, suggesting that PAR-3 may be involved in thrombosis or mitogenesis. Thrombin receptor and PAR-4 appear to account for most thrombin signaling in platelets. Activation of PAR-2 in vitro is induced by trypsin, suggesting that PAR-2 is not an alternative thrombin receptor. Cytokines including TNF-α and IL-1β increase PAR-2 expression, indicating PAR-2 involvement in the acute inflammatory response.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Thrombin R (G17) polyclonal antibody detects endogenous levels of Thrombin R protein\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Thrombin R (G17) pAb at 1:500 dilution\u003cbr\u003e\nLane1:PC3 whole cell lysate(40ug)\u003cbr\u003e\nLane2:U-87MG whole cell lysate(40ug)\u003cbr\u003e\nLane3:The Brian tissue lysate of Rat(10ug)\u003cbr\u003e\nLane4:The Brain tissue lysate of Mouse(20ug)\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 47 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor; F2R; CF2R; PAR1; TR\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the N-terminal of Human Thrombin R.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42910476599475,"sku":"BS1856-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42910476632243,"sku":"BS1856-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_18620fc7-2850-4528-af97-d2b4856c848a.jpg?v=1703093183","url":"https:\/\/afsbio.com\/products\/thrombin-r-g17-polyclonal-antibody-bs1856","provider":"AFSBio Inc.","version":"1.0","type":"link"}