{"product_id":"tnf-alpha-monoclonal-antibody-mb65982","title":"TNF alpha Monoclonal Antibody - MB65982","description":"\u003cp\u003eTNF alpha Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μl, 100μl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB65982-50, MB65982-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1, 2). Membrane-bound TNF-α is cleaved by the metalloprotease TACE\/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellμlar response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and resμlts in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk1\/2, p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death (1, 2). TNF-α plays a key regμlatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tubercμlosis (3).\u003c\/p\u003e\n\n\u003cp\u003eCategory: Primary Antibody\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: TNFA, TNFSF2, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a\u003c\/p\u003e\n\n\u003cp\u003eClonality: Monoclonal\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein corresponding to human TNF alpha.\u003c\/p\u003e\n\n\u003cp\u003eDilution: WB (1\/1000 - 1\/2000), IHC (1\/50 - 1\/200)\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of TNF alpha protein.\u003c\/p\u003e\n\n\u003cp\u003eMolecular Weight: ~ 17 kDa\u003c\/p\u003e\n\n\u003cp\u003ePathway: Death Receptor Signaling, Inhibition of Apoptosis,\u003c\/p\u003e \n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSwissProt: P01375\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Bioworld","offers":[{"title":"50μl","offer_id":45946394771635,"sku":"MB65982-50","price":274.5,"currency_code":"CAD","in_stock":true},{"title":"100μl","offer_id":45946394804403,"sku":"MB65982-100","price":457.5,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Bioworld_Antibody_Image_6ca33534-2486-4e91-ac84-9e0d75778258.jpg?v=1788363539","url":"https:\/\/afsbio.com\/products\/tnf-alpha-monoclonal-antibody-mb65982","provider":"AFSBio Inc.","version":"1.0","type":"link"}