{"product_id":"topoisomerase-i-monoclonal-antibody-mb11707","title":"Topoisomerase I Monoclonal Antibody-MB11707","description":"\u003cp\u003eTopoisomerase I Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB11707-50, MB11707-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11387\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-F, IHC-P, ICC\/IF\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IF: 1\/50-1\/200\u003c\/p\u003e  \n\n\u003cp\u003eBackground: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 91 kDa; Observed MW: 91 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Topoisomerase I in K562, rat Brain, C6, 3T3, Hela lysates using Topoisomerase I antibody., Immunocytochemistry analysis of Topoisomerase I in Hela using Topoisomerase I antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Topoisomerase I antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: TOP1; DNA topoisomerase 1; DNA topoisomerase I\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human Topoisomerase I\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004746170547,"sku":"MB11707-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004746203315,"sku":"MB11707-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_99a5e7bc-82d5-437e-8879-a699640ab553.jpg?v=1706445398","url":"https:\/\/afsbio.com\/products\/topoisomerase-i-monoclonal-antibody-mb11707","provider":"AFSBio Inc.","version":"1.0","type":"link"}