{"product_id":"topoisomerase-i-polyclonal-antibody-bz16686","title":"Topoisomerase I Polyclonal Antibody-BZ16686","description":"\u003cp\u003eTopoisomerase I Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BZ16686-50, BZ16686-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P11387\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eTopoisomerase I Polyclonal Antibody-BZ16686\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-P, ICC\/IF, FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000 IHC: 1\/50-1\/100 IF: 1\/50-1\/200 FC: 1\/50-1\/100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 91 kDa; Observed MW: 91 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of TOP1 in JurkatLysates using Topoisomerase I antibody., Immunohistochemistry analysis of paraffin-embedded Human colon using TOP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: TOP1; DNA topoisomerase 1; DNA topoisomerase I\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthesized peptide derived from human TOP1\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957328089267,"sku":"BZ16686-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957328122035,"sku":"BZ16686-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_aac42186-4e3d-4d51-b8b2-97beb81d4cb0.jpg?v=1705144909","url":"https:\/\/afsbio.com\/products\/topoisomerase-i-polyclonal-antibody-bz16686","provider":"AFSBio Inc.","version":"1.0","type":"link"}