{"product_id":"torc2-phospho-s171-polyclonal-antibody-bs64579","title":"TORC2 (Phospho-S171) Polyclonal Antibody-BS64579","description":"\u003cp\u003eTORC2 (Phospho-S171) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS64579-50, BS64579-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q53ET0\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling. The protein CRTC2 (CREB-regulated transcription coactivator 2)\/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways. In quiescent cells, CRTC2\/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2\/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2\/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2\/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting. CRTC2\/TORC2-related proteins CRTC1\/TORC1 and CRTC3\/TORC3 also act as CREB co-activators. CRTC1\/TORC1, CRTC2\/TORC2 and CRTC3\/TORC3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats. CRTC1\/TORC1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions. When these cells are exposed to cAMP or a calcium activator, CRTC1\/TORC1 is dephosphorylated and translocates into the nucleus. CRTC1\/TORC1 is essential for energy balance and fertility.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: TORC2 (Phospho-S171) polyclonal antibody detects endogenous levels of TORC2 protein only when phosphorylated at Ser171.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 87, 73 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western Blot (WB) analysis of TORC2 (Phospho-S171) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane A: Hela whole cell lysate\u003cbr\u003e\nLane B: HEK293T whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2; TORC-2; Transducer of CREB protein 2; CRTC2; TORC2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human TORC2 around the phosphorylation site of Serine 171.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42923464065203,"sku":"BS64579-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42923464097971,"sku":"BS64579-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_a5b53a35-246e-47b7-a676-b28b5adb3cc1.jpg?v=1703849988","url":"https:\/\/afsbio.com\/products\/torc2-phospho-s171-polyclonal-antibody-bs64579","provider":"AFSBio Inc.","version":"1.0","type":"link"}