{"product_id":"total-cholesterol-and-cholesteryl-ester-fluorometric-assay-kit-e-bc-f032","title":"Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit - E-BC-F032","description":"\u003cp\u003eTotal Cholesterol and Cholesteryl Ester Fluorometric Assay Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-BC-F032-48, E-BC-F032-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Glycolysis and Lipid Metabolism\u003c\/p\u003e\n\n\u003cp\u003eResult Type: Quantitative\u003c\/p\u003e\n\n\u003cp\u003eDetection Instrument: Fluorescence Microplate Reader\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, plasma, animal tissue, cells\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 20 min\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Fluorescence method\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.12 μmol\/L\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.12-30 μmol\/L\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE) . Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.\u003c\/p\u003e\n\n\u003cp\u003eInter CV: 7.3\u003c\/p\u003e\n\n\u003cp\u003eIntra CV: 1.7\u003c\/p\u003e\n\n\u003cp\u003eStorage: This product can be stored at -20°C for 12 months with shading light.\u003c\/p\u003e\n\n\u003cp\u003eShipping: Ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"96T","offer_id":43047602782387,"sku":"E-BC-F032-96","price":560.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43047602815155,"sku":"E-BC-F032-48","price":392.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Screenshot_2024-02-12_153513_16da112c-7e71-4d99-bc9f-1338a543fbd3.png?v=1707825709","url":"https:\/\/afsbio.com\/products\/total-cholesterol-and-cholesteryl-ester-fluorometric-assay-kit-e-bc-f032","provider":"AFSBio Inc.","version":"1.0","type":"link"}