{"product_id":"tph1-phospho-s58-polyclonal-antibody-bs64532","title":"TPH1 (Phospho-S58) Polyclonal Antibody-BS64532","description":"\u003cp\u003eTPH1 (Phospho-S58) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS64532-50, BS64532-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P17752\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500~1:1000 IHC:1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: Phenylalanine hydroxylase (PAH), tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) comprise a small family of monooxygenases that use tetrahydropterine as a cofactor during the catabolism of aromatic L-amino acids. PAH, TH and TPH all contain catalytic domains with an amino-terminal regulatory domain and a short carboxy-terminal tetramerization domain. Each of these enzymes also contains a single ferrous iron atom, which is bound to two histidines and a glutamate and is likely to be involved in the formation of the hydroxylating intermediate. TPH is the first and rate-limiting step in the biosynthesis of serotonin in the central nervous system and melatonin in the pineal gland.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: TPH1 (Phospho-S58) polyclonal antibody detects endogenous levels of TPH1 protein only when phosphorylated at Ser58.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 51 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of TPH1 (Phospho-S58) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane A: HEK293T whole cell lysate\u003cbr\u003e\nLane B: THP-1 whole cell lysate\u003cbr\u003e\nLane C: MCF-7 whole cell lysate\u003cbr\u003e\nLane D: SGC7901 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; TPH1; TPH; TPRH; TRPH\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human TPH1 around the phosphorylation site of Serine 58.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044029300915,"sku":"BS64532-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044029333683,"sku":"BS64532-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_ad9bd233-5da3-46bd-8b17-6cd5542c490a.jpg?v=1707738677","url":"https:\/\/afsbio.com\/products\/tph1-phospho-s58-polyclonal-antibody-bs64532","provider":"AFSBio Inc.","version":"1.0","type":"link"}