For pricing in CAD please contact us

Trastuzumab (Herceptin) Immunogenicity ELISA

Trastuzumab (Herceptin) Immunogenicity ELISA

Regular price
$1,279.98
Regular price
Sale price
$1,279.98
Unit price
per 
Availability
Sold out

Trastuzumab ELISA Herceptin ELISA immunogenicity Biosimilars

 

INTRODUCTION
Trastuzumab (Herceptin® ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2). Repeated dosing of Trastuzumab has been reported to induce a neutralizing antibody response resulting in reduce efficacy of Trastuzumab. This kit allows researchers to qualitatively monitor the presence of anti-Trastuzumab antibodies in biological matrices.

PRINCIPLE OF THE ASSAY
This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount of anti-Trastuzumab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Trastuzumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.

Product Properties

CATALOGUE NUMBER: EL-141-201

DETECTION METHOD
Peroxidase / OD450

STORAGE AND STABILITY
Stable at -20°C for 1 year

SAFETY WARNINGS AND PRECAUTIONS
Research Use Only. Follow instructions.

PRECISION
Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.

DETECTION LIMIT
31.3ng/ml

EACH KIT INCLUDES:

  • Coated microtiter plate, 96 wells
  • QC samples - 4x50ul
  • 10X wash buffer - 25ml
  • Assay buffer - 50ml
  • 1000X secondary antibody - 17ul
  • 1000X detection reagent - 17ul
  • TMB - 12ml
  • TMB stop solution - 12ml
  • Plate sealers - 3

Download Manual Here