{"product_id":"trim25-rabbit-monoclonal-antibody-mb66399","title":"TRIM25 Rabbit Monoclonal Antibody-MB66399","description":"\u003cp\u003eTRIM25 Rabbit Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB66399-50, MB66399-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q14258\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB (1\/500 - 1\/1000), IF\/ICC (1\/50 - 1\/100), IP (1\/10 - 1\/50)\u003c\/p\u003e\n\n\u003cp\u003eBackground: Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase .\u003cbr\u003e\nInvolved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1.\u003cbr\u003e\nMediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection.\u003cbr\u003e\nMediates 'Lys-63'-linked polyubiquitination of IFIH1.\u003cbr\u003e\nPromotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway .\u003cbr\u003e\nMediates estrogen action in various target organs.\u003cbr\u003e\nMediates the ubiquitination and subsequent proteasomal degradation of ZFHX3.\u003cbr\u003e\nPlays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity).\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was purified by immunogen affinity chromatography.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Recognizes endogenous levels of TRIM25 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 70 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of TRIM25 expression in K562 (A), rat brain (B), C6 (C), Hela (D) whole cell lysates., Immunofluorescent analysis of TRIM25 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: EFP; RNF147; ZNF147; E3 ubiquitin\/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin\/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide of human TRIM25\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989540999347,"sku":"MB66399-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989541032115,"sku":"MB66399-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5d438155-4609-49fa-b8c8-7ae00c215416.jpg?v=1706095043","url":"https:\/\/afsbio.com\/products\/trim25-rabbit-monoclonal-antibody-mb66399","provider":"AFSBio Inc.","version":"1.0","type":"link"}