{"product_id":"trim28-c-terminus-monoclonal-antibody-mb0014","title":"TRIM28 (C-terminus) Monoclonal Antibody-MB0014","description":"\u003cp\u003eTRIM28 (C-terminus) Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB0014-50, MB0014-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q13263\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000\u003cbr\u003e\nICC: 1:200\u003cbr\u003e\nIHC: 1:500\u003c\/p\u003e \n\n\u003cp\u003eBackground: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A\/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of TRIM28(C-terminus) and does not cross-react with related proteins\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Predicted band size: 100KDa \u003cbr\u003e\nObserved band size: 100KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot detection of TRIM28 (C-terminus) in 293T and HepG2 cell lysates using TRIM28 (C-terminus) antibody (1:1000 diluted)., IHC of paraffin-embedded human Spleen using anti- TRIM28 (C-terminus) diluted 1\/500-1\/1000., Immunocytochemistry of HeLa cells using anti- TRIM28 (C-terminus) antibody diluted 1:200.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: E3 SUMO-protein ligase TRIM28; \u003cbr\u003e\nKRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; KAP1; TF1B; RNF96; TIF1B; TRIM28\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to the C-terminus of Human TRIM28.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997588132019,"sku":"MB0014-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997588164787,"sku":"MB0014-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0fba219a-3412-42d5-aefe-0e545820002d.jpg?v=1706270781","url":"https:\/\/afsbio.com\/products\/trim28-c-terminus-monoclonal-antibody-mb0014","provider":"AFSBio Inc.","version":"1.0","type":"link"}