{"product_id":"trimethyl-histone-h3-lys27-monoclonal-antibody-mb11844","title":"TriMethyl-Histone H3 (Lys27) Monoclonal Antibody-MB11844","description":"\u003cp\u003eTriMethyl-Histone H3 (Lys27) Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB11844-50, MB11844-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P68431\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC-F, IHC-P, ICC\/IF, IP, , ChIP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1\/500-1\/1000  IHC: 1\/50-1\/100  IF: 1\/50-1\/200  IP: 1\/20  ChIP: 1\/20\u003c\/p\u003e  \n\n\u003cp\u003eBackground: H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Affinity Purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: IgG\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Histone H3 in C6, Jurkat lysates using TriMethyl-Histone H3 antibody., Immunocytochemistry analysis of TriMethyl-Histone H3 in Hela using TriMethyl-Histone H3 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TriMethyl-Histone H3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: H3K27me3; H3 histone; HIST1H3A; Histone cluster 1; H3a\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic methyl-peptide corresponding to residues surrounding Lys27 of human Histone H3\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Methylated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43004748267699,"sku":"MB11844-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43004748300467,"sku":"MB11844-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_bacf6ebe-501a-4fa9-be66-01f245070ca3.jpg?v=1706445472","url":"https:\/\/afsbio.com\/products\/trimethyl-histone-h3-lys27-monoclonal-antibody-mb11844","provider":"AFSBio Inc.","version":"1.0","type":"link"}