{"product_id":"ube2e2-polyclonal-antibody-bs76787","title":"UBE2E2 Polyclonal Antibody-BS76787","description":"\u003cp\u003eUBE2E2 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers:  BS76787-50, BS76787-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q96LR5\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Ube2E2 is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for ubiquitination of protein substrates.  It shares 96% identical amino acid sequence with Ube2E1 and Ube2E3 with the exception of the N-terminal extension that has little in common with the other two E2s. Ube2E2 interacts with ARA54 and RNF8.  The RNF8\/ Ube2E2 interaction is found to catalyze K48-linked poly-ubiquitination.  Ube2E2 may play a role in ubiquitination and subsequent proteasomal degradation of the E3 Ub ligase Parkin.\u003c\/p\u003e \n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e \n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using UBE2E2 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% nonfat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Enhanced Kit.\u003cbr\u003e\nExposure time: 180s.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 22KDa\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: UBE2E2; UBCH8\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human UBE2E2(NP_689866.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42924596002995,"sku":"BS76787-50","price":334.88,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42924596035763,"sku":"BS76787-100","price":576.38,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_75e0e9c8-93a0-4604-9e31-37e78bb07d9c.jpg?v=1703936453","url":"https:\/\/afsbio.com\/products\/ube2e2-polyclonal-antibody-bs76787","provider":"AFSBio Inc.","version":"1.0","type":"link"}