{"product_id":"ube3b-rabbit-pab-es7927","title":"UBE3B rabbit pAb - ES7927","description":"\u003cp\u003eUBE3B rabbit pAb\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES7927-50, ES7927-100\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eBackground: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [pro\u003c\/p\u003e\n\n\u003cp\u003eAlternate Name: UBE3B; Ubiquitin-protein ligase E3B\u003c\/p\u003e\n\n\u003cp\u003eSource: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB; IHC; IF; ELISA\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. Immunohistochemistry: 1\/100 - 1\/300. ELISA: 1\/10000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human; Rat; Mouse;\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human UBE3B. AA range:581-630\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 123kD\u003c\/p\u003e\n\n\u003cp\u003eHuman Gene ID: 89910\u003c\/p\u003e\n\n\u003cp\u003eHuman SWISS Prot NO: Q7Z3V4\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: nucleus, cytoplasm,\u003c\/p\u003e \n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"50μL","offer_id":45202364530867,"sku":"ES7927-50","price":207.2,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":45202364563635,"sku":"ES7927-100","price":347.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_9c59452a-063f-403c-8625-db5738a539f4.png?v=1767127533","url":"https:\/\/afsbio.com\/products\/ube3b-rabbit-pab-es7927","provider":"AFSBio Inc.","version":"1.0","type":"link"}