{"product_id":"uchl5-polyclonal-antibody-bs71027","title":"UCHL5 Polyclonal Antibody-BS71027","description":"\u003cp\u003eUCHL5 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50 µl, 100 µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS71027-50, BS71027-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q9Y5K5\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IF\/ICC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Uch37 is one of the deubiquitinating enzymes of the human 26S proteasome, belongs to the Ub C-terminal hydrolases. Uch37 was found to bind both Adrm1 and S5a subunits of the 26S proteasome.  Uch37 likely contributes to the Ub chain trimming activity of the 26S proteasome that shortens a polyUb chain from the distal end of the chain.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Polyclonal Antibodies\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 38KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of various lysates, using UCHL5 antibody at 1:433 dilution. \u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. \u003cbr\u003e\nLysates\/proteins: 25ug per lane. \u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST. \u003cbr\u003e\nDetection: ECL Basic Kit. \u003cbr\u003e\nExposure time: 15s., Immunofluorescence analysis of NIH\/3T3 cells using UCHL5 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: UCHL5; CGI-70; INO80R; UCH-L5; UCH37\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human UCHL5(NP_001186190.1).\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42968530976947,"sku":"BS71027-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42968531009715,"sku":"BS71027-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_bcdc8dc3-677a-4579-9b8d-fecb20cf96df.jpg?v=1705430412","url":"https:\/\/afsbio.com\/products\/uchl5-polyclonal-antibody-bs71027","provider":"AFSBio Inc.","version":"1.0","type":"link"}