{"product_id":"ucp3-polyclonal-antibody-bs80119","title":"UCP3 Polyclonal Antibody-BS80119","description":"UCP3 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS80119-50, BS80119-100\n\nProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\n\nSwiss-Prot: P55916\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, IF\/ICC\n\nAll Applications: WB,1:500 - 1:2000   |   IF\/ICC,1:50 - 1:200\n\nBackground: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: Unmodification \n\nBioworld Molecular Weight: 34kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Western blot analysis of extracts of various cell lines, using UCP3 Rabbit pAb at 1:1000 dilution.\n Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\n Lysates\/proteins: 25ug per Lane.\n Blocking buffer: 3% non-fat dry milk in TBST.\n Detection: ECL Basic Kit.\n Exposure time: 90s., Immunofluorescence analysis of C6 cells using UCP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using UCP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH\/3T3 cells using UCP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.\n\nAlternative Name: UCP3; SLC25A9\n\nImmunogen: Recombinant fusion protein of human UCP3(P55916).\n\nConjugate: Unconjugated\n\nModification: Unmodified ","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43050210558131,"sku":"BS80119-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43050210590899,"sku":"BS80119-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_6c255d7f-a476-4b20-a13d-b7794bf1d705.jpg?v=1707913179","url":"https:\/\/afsbio.com\/products\/ucp3-polyclonal-antibody-bs80119","provider":"AFSBio Inc.","version":"1.0","type":"link"}