{"product_id":"usp9x-polyclonal-antibody-bs9954m","title":"USP9x Polyclonal Antibody-BS9954M","description":"\u003cp\u003eUSP9x Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS9954M-50, BS9954M-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q93008\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:1000-1:2000\u003cbr\u003e\nIHC: 1:50-1:200\u003cbr\u003e\nIF: 1:50-1:200\u003cbr\u003e\nFC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-48'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Essential component of TGF-beta\/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Deubiquitinates alkylation repair enzyme ALKBH3. OTUD4 recruits USP7 and USP9X to stabilize ALKBH3, thereby promoting the repair of alkylated DNA lesions. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5\/survivin to mitotic centromeres. Involved in axonal growth and neuronal cell migration.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of USP9x and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 50 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of USP9x Rabbit mAb at 1:1000 dilution\u003cbr\u003e\n Lane 1: SiHa whole cell lysate\u003cbr\u003e\n Lane 2: A549 whole cell lysate\u003cbr\u003e\n Lane 3: 293 whole cell lysate\u003cbr\u003e\n Lane 4: The colon tissue lysate of Mouse, Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-USP9X antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins., ICC staining USP9X in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS., Flow cytometric analysis of 293T cells with USP9X antibody at 1\/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Probable ubiquitin carboxyl-terminal hydrolase FAF-X; Deubiquitinating enzyme FAF-X; Fat facets in mammals; hFAM; Fat facets protein-related, X-linked\u003cbr\u003e\nUbiquitin thioesterase FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; USP9X; DFFRX; FAM; USP9\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein within human USP9X aa 1-200.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43058777587891,"sku":"BS9954M-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43058777620659,"sku":"BS9954M-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_87ed9bde-06a4-4c39-9a9a-e562ae091e3b.jpg?v=1708172195","url":"https:\/\/afsbio.com\/products\/usp9x-polyclonal-antibody-bs9954m","provider":"AFSBio Inc.","version":"1.0","type":"link"}