{"product_id":"v5-tag-monoclonal-antibody-mb8023","title":"V5-tag Monoclonal Antibody-MB8023","description":"\u003cp\u003eV5-tag Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB8023-50, MB8023-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Transfected\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IP,\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:5000~1:20000\u003cbr\u003e\nIP：1:100~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: V5 mAb detects endogenous levels of V5 protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 95 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of V5 mAb at 1:5000 dilution\u003cbr\u003e\n Lane 1: HEK293T whole cell lysate (40ug)\u003cbr\u003e\n Lane 2: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (10ug)\u003cbr\u003e\n Lane 3: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (20ug)\u003cbr\u003e\n Lane 4: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (30ug)\u003cbr\u003e\n Lane 5: HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate (40ug), Immunoprecipitation of HEK293T transfected with pcDNA3.1-MCM5-V5-His（48h）whole cell lysate using V5 mAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4). Lane 1 is 20% input. The western blot was probed using V5 mAb #MB8023. “↑”（supernatant); “↓”（deposition）\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: V5tag\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to V5 tag.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42989583564979,"sku":"MB8023-50","price":263.54,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42989583597747,"sku":"MB8023-100","price":424.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_9ff4b449-d52d-4f71-99dd-4e491a743357.jpg?v=1706095753","url":"https:\/\/afsbio.com\/products\/v5-tag-monoclonal-antibody-mb8023","provider":"AFSBio Inc.","version":"1.0","type":"link"}