{"product_id":"vc-vitamin-c-elisa-kit-elk8125","title":"VC (Vitamin C) ELISA Kit - ELK8125","description":"\u003cp\u003eVC (Vitamin C) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK8125-48, ELK8125-96, ELK8125-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: AA; L-Ascorbic Acid; Ascorbate\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 184 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 625-40000 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Infection immunity; Nutrition metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VC in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212362612915,"sku":"ELK8125-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212362645683,"sku":"ELK8125-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200791011507,"sku":"ELK8125-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_123ddeff-14f1-44e9-a874-4a9b5fd3d6ce.png?v=1767063893","url":"https:\/\/afsbio.com\/products\/vc-vitamin-c-elisa-kit-elk8125","provider":"AFSBio Inc.","version":"1.0","type":"link"}