{"product_id":"vcam1-polyclonal-antibody-bs66143","title":"VCAM1 Polyclonal Antibody-BS66143","description":"VCAM1 Polyclonal Antibody\n\nSizes: 50µl, 100µl\n\nCatalogue Numbers: BS66143-50, BS66143-100\n\nProduct: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.\n\nSwiss-Prot: P19320\n\nHost: Rabbit\n\nReactivity: Human, Mouse, Rat\n\nApplications: WB, ELISA IHC IF\n\nAll Applications: WB: 1:500-1:2000\nELISA: 1:5000-1:10000\nIHC: 1:100-1:500\nIF: 1:100-1:500\n\nBackground: The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the B2α1 and B2αM integrins. PECAM-1 (platelet\/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM) -like protein and is deleted in 66% of patients with Kallmann's syndrome, anosmia with secondary hypogonadism .\n\nPurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\n\nStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\n\nSpecificity: VCAM1 polyclonal antibody detects endogenous levels of VCAM1 protein.\n\nBioworld Molecular Weight: ~ 81, 110 kDa\n\nNote: For research use only, not for use in diagnostic procedure.\n\nExtra Notes: Primary: Anti-VCAM-1 at 1\/1000 dilution, Primary: Anti-VCAM-1 at 1\/300 dilution, Tissue\/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;\n Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min;\n Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.\n\nAlternative Name: Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; L1CAM\n\nImmunogen: KLH conjugated synthetic peptide derived from mouse VCAM-1: 640-739\/739 \u003cextracellular\u003e\n\nConjugate: Unconjugated\n\nModification: Unmodified\u003c\/extracellular\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42997560705203,"sku":"BS66143-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42997560737971,"sku":"BS66143-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_2c70eb4f-9f8f-4c90-b347-75512e17ee82.jpg?v=1706270442","url":"https:\/\/afsbio.com\/products\/vcam1-polyclonal-antibody-bs66143","provider":"AFSBio Inc.","version":"1.0","type":"link"}