{"product_id":"vimentin-mouse-monoclonal-antibody-mb9006","title":"Vimentin Mouse Monoclonal Antibody-MB9006","description":"\u003cp\u003eVimentin Mouse Monoclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: MB9006-50, MB9006-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Mouse IgG1, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P08670\u003c\/p\u003e\n\n\u003cp\u003eHost: Mouse\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:2000-1:5000 ICC: 1:200\u003cbr\u003e\nIHC: 1:200 FC: 1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein A affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This antibody detects endogenous levels of Vimentin and does not cross-react with related proteins.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 54 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Vimentin Mouse mAb at 1:2000 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate\u003cbr\u003e\n Lane 2: Hela whole cell lysate\u003cbr\u003e\n Lane 3: NIH\/3T3 whole cell lysate\u003cbr\u003e\n Lane 4: The embryonic stem cell lysate of Mouse, Immunohistochemical analysis of paraffin-embedded human lung tissue using vimentin antibody. Counter stained with hematoxylin., ICC staining vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Vimentin; VIM\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of Vimentin.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":43044036182195,"sku":"MB9006-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":43044036214963,"sku":"MB9006-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_51c89130-a776-4d73-a984-9443ad8df716.jpg?v=1707738836","url":"https:\/\/afsbio.com\/products\/vimentin-mouse-monoclonal-antibody-mb9006","provider":"AFSBio Inc.","version":"1.0","type":"link"}