{"product_id":"vimentin-polyclonal-antibody-bs91440","title":"Vimentin Polyclonal Antibody-BS91440","description":"\u003cp\u003eVimentin Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS91440-50, BS91440-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P08670(Human) P20152(Mouse) P31000(Rat)\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC\/IF, IHC, IP, , FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:1,000-1:5,000\u003cbr\u003e\nICC:1:100-1:500\u003cbr\u003e\nIHC:1:100-1:500\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, a-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: ProA affinity purified\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Vimentin polyclonal antibody detects endogenous levels of Vimentin protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 54 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Vimentin on different lysates using anti-Vimentin antibody at 1\/1,000 dilution. \u003cbr\u003e\n Positive control:\u003cbr\u003e\n Lane 1: Hela\u003cbr\u003e\n Lane 2: A549, ICC staining Vimentin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100\/PBS.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: VIM, CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME, Vimentin,\u003c\/p\u003e \n\n\u003cp\u003eImmunogen: recombinant protein\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42987062395059,"sku":"BS91440-50","price":424.94,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42987062427827,"sku":"BS91440-100","price":655.54,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_b53fd3c8-ea53-4fbc-92d6-59db6e7a5cf5.jpg?v=1706008224","url":"https:\/\/afsbio.com\/products\/vimentin-polyclonal-antibody-bs91440","provider":"AFSBio Inc.","version":"1.0","type":"link"}